Product Name

ACTA2/smooth muscle actin antibody

Documents

Description

ACTA2/smooth muscle actin Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, mouse brown adipose tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat heart tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in human heart tissue, human colon tissue, human skeletal muscle tissue, human small intestine tissue. Observed molecular weight by Western-blot: 42 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human, Mouse, Rat; other species not tested.

Alternative names

a SMA antibody; AAT6 antibody; ACTA2 antibody; ACTA2 Smooth muscle Actin antibody; ACTA2 specific antibody; Actin antibody; aortic smooth muscle antibody; ACTSA antibody; ACTVS antibody; Alpha actin 2 antibody; Alpha SMA antibody; a-SMA antibody; α SMA antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ACTA2/smooth muscle actin recombinant protein (Accession Number: NM_001406463). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:200-1:1000

Validations

Immunohistochemical of paraffin-embedded human heart using Catalog No:107706(ACTA2 antibody) at dilution of 1:500 (under 10x lens)

mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107706(ACTA2 antibody) at dilution of 1:1000

Immunohistochemical of paraffin-embedded human heart using Catalog No:107706(ACTA2 antibody) at dilution of 1:500 (under 40x lens)

IP Result of anti-a-SMA specific (IP:Catalog No:107706, 4ug; Detection:Catalog No:107706 1:1000) with mouse heart tissue lysate 3200ug.

Background

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.

References

Fan YF, Zhang R, Jiang X. The phosphodiesterase-5 inhibitor vardenafil reduces oxidative stress while reversing pulmonary arterial hypertension. Cardiovascular research. 99(3):395-403. 2013.
He L, Zhou X, Qu C. Musashi2 predicts poor prognosis and invasion in hepatocellular carcinoma by driving epithelial-mesenchymal transition. Journal of cellular and molecular medicine. 18(1):49-58. 2014.
Chen HH, Weng BQ, Cheng KJ, Liu HY, Wang SQ, Lu YY. Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth. Vascular cell. 6(1):4. 2014.
He Y, Wu YT, Huang C. Inhibitory effects of long noncoding RNA MEG3 on hepatic stellate cells activation and liver fibrogenesis. Biochimica et biophysica acta. 1842(11):2204-15. 2014.
Liang C, Li X, Zhang L, Cui D, Quan X, Yang W. The anti-fibrotic effects of microRNA-153 by targeting TGFBR-2 in pulmonary fibrosis. Experimental and molecular pathology. 99(2):279-85. 2015.

ACTA2/smooth muscle actin antibody

Product Information

Related Products / Services