Cart 2

ACTA2/smooth muscle actin antibody

Cat.#: 107705

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    ACTA2/smooth muscle actin antibody
  • Documents
  • Description
    ACTA2/smooth muscle actin Rabbit Polyclonal antibody. Positive FC detected in C2C12 cell. Positive IF detected in C2C12 cell. Positive IHC detected in human hysteromyoma tissue, human heart tissue, human liver cancer tissue, human liver tissue, human skeletal muscle tissue. Positive IP detected in mouse liver tissue. Positive WB detected in human placenta tissue, HEK-293 cells, human bladder tissue, K-562 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse ovary tissue, rat heart tissue. Observed molecular weight by Western-blot: 43 kDa
  • Tested applications
    ELISA, IHC, IP, WB, FC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    a SMA antibody; AAT6 antibody; ACTA2 antibody; ACTA2 Smooth muscle Actin antibody; Actin antibody; aortic smooth muscle antibody; Actin antibody; aortic smooth muscle Actin antibody; ACTSA antibody; ACTVS antibody; Alpha actin 2 antibody; Alpha SMA antibody; a-SMA antibody; α SMA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ACTA2/smooth muscle actin recombinant protein (Accession Number: NM_001406463). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107705(ACTA2 antibody) at dilution of 1:1000

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107705(ACTA2 antibody) at dilution of 1:1000

    IP Result of anti-ACTA2 (IP:Catalog No:107705, 3ug; Detection:Catalog No:107705 1:1000) with mouse liver tissue lysate 6000ug.

    IP Result of anti-ACTA2 (IP:Catalog No:107705, 3ug; Detection:Catalog No:107705 1:1000) with mouse liver tissue lysate 6000ug.

    Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:107705(ACTA2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:107705(ACTA2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:107705(ACTA2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:107705(ACTA2 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of (-20oc Ethanol) fixed C2C12 cell using Catalog No:107705(ACTA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed C2C12 cell using Catalog No:107705(ACTA2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 C2C12 cell were stained with 0.2ug ACTA2 antibody (Catalog No:107705, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 C2C12 cell were stained with 0.2ug ACTA2 antibody (Catalog No:107705, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
  • References
    • Liu Y, Dong W, Chen L. BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2. The Journal of biological chemistry. 279(36):37436-44. 2004.
    • Zhang W, Miao J, Ma C, Han D, Zhang Y. β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells. Peptides. 36(2):186-91. 2012.
    • He Y, Huang C, Sun X, Long XR, Lv XW, Li J. MicroRNA-146a modulates TGF-beta1-induced hepatic stellate cell proliferation by targeting SMAD4. Cellular signalling. 24(10):1923-30. 2012.
    • Yu L, Guo W, Zhao S, Wang F, Xu Y. Fusion between cancer cells and myofibroblasts is involved in osteosarcoma. Oncology letters. 2(6):1083-1087. 2011.
    • Zhang SS, Gong ZJ, Li WH, Wang X, Ling TY. Antifibrotic effect of curcumin in TGF-β 1-induced myofibroblasts from human oral mucosa. Asian Pacific journal of cancer prevention : APJCP. 13(1):289-94. 2012.
    • Bian EB, Huang C, Wang H. Repression of Smad7 mediated by DNMT1 determines hepatic stellate cell activation and liver fibrosis in rats. Toxicology letters. 224(2):175-85. 2014.
    • Sun X, He Y, Ma TT, Huang C, Zhang L, Li J. Participation of miR-200a in TGF-β1-mediated hepatic stellate cell activation. Molecular and cellular biochemistry. 388(1-2):11-23. 2014.
    • Wang L, Jiang M, Duan D. Hyperthermia-conditioned OECs serum-free-conditioned medium induce NSC differentiation into neuron more efficiently by the upregulation of HIF-1 alpha and binding activity. Transplantation. 97(12):1225-32. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"