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Product Name
acid phosphatase, prostate antibody
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Description
acid phosphatase, prostate Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human prostate cancer tissue, human prostate hyperplasia tissue. Positive WB detected in PC-3 cells, COLO 320 cells, MCF7 cells, mouse lung tissue, mouse testis tissue. Observed molecular weight by Western-blot: 50-60 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
acid phosphatase antibody; prostate antibody; ACP 3 antibody; ACP3 antibody; ACPP antibody; PAP antibody; Prostatic acid phosphatase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of acid phosphatase, prostate recombinant protein (Accession Number: NM_001134194). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:107738(ACPP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107738(ACPP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:107738(ACPP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:107738(ACPP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
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References
- Bae H, Lim W, Bae SM, Bazer FW, Choi Y, Song G. Avian prostatic acid phosphatase: estrogen regulation in the oviduct and epithelial cell-derived ovarian carcinomas. Biology of reproduction. 91(1):3. 2014.
- Lim W, Bae H, Sohn JY, Jeong W, Kim SH, Song G. Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats. Biochemical and biophysical research communications. 456(1):421-7. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"