2019-nCoV S1 Protein (Mammalian, C-6His)

2019-nCoV S1 Protein (Mammalian, C-6His)

• DataSheet
• Material Safety Data Sheets (MSDS)

Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It is been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

S1 protein; 2019-nCoV S1 protein; coronavirus S1 Protein; cov S1 Protein

QHD43416.1

Human Cells

The target gene encoding Val16-Arg685 is expressed with a 6His tag at the C-terminus.

Severe acute respiratory syndrome coronavirus 2

100-130kDa, reducing conditions

Greater than 95% as determined by reducing SDS-PAGE.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) is 200-600 ng/mL.