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Product Name
14-3-3 sigma antibody
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Description
14-3-3 sigma Mouse Monoclonal antibody. Positive WB detected in MCF-7 cells, A431 cells, HeLa cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human skin tissue, human breast hyperplasia tissue. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
14 3 3 protein sigma antibody; HME1 antibody; SFN antibody; stratifin antibody; YWHAS antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of 14-3-3 sigma recombinant protein (Accession Number: NM_006142). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107551(SFN Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:107551(SFN Antibody) at dilution of 1:2000 (under 10x lens).
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:107551(SFN Antibody) at dilution of 1:2000 (under 40x lens).
Immunofluorescent analysis of MCF-7 cells using Catalog No:107551(SFN Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
SFN represents stratifin
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