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Codon Optimization Tool (ExpOptimizer)

The ExpOptimizer is developed for the high expression of any target proteins in any mainstream expression hosts. Its comprehensive codon optimization algorithm considerate dozens of key factors of gene transcription and translation *.

Please input the CDS sequence of your gene and the length must be multiples of 3 if you input DNA/RNA sequence.

0. Sequence Type:

1. Sequence (Paste the raw sequence, not fasta format):

Full length:0

2. Select Expression Host:

3. Select the restriction enzymes to exclude:



Optimization Parameters

  • Codon usage bias
  • 5' region optimization
  • DNA repeats
  • mRNA secondary structure
  • GC content
  • SD sequence
  • Exclude restriction sites specified

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  • Yufeng Xie, Lijun Qin, Degang Zhao, Preparation of gutta-percha through fermentative degradation of Eucommia ulmoides Oliver leaves by recombinant yeast expressing cellulases from Trichoderma reesei, Industrial Crops and Products, Volume 192, 2023, 115830, ISSN 0926-6690
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  • Afshari E, Cohan RA, Sotoodehnejadnematalahi F, Mousavi SF. In-silico design and evaluation of an epitope-based serotype-independent promising vaccine candidate for highly cross-reactive regions of pneumococcal surface protein A. J Transl Med. 2023 Jan 10;21(1):13.
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