To prepare L Streptomycin Stock Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Streptomycin sulfate | 100 g | 100 mg/mL | C42H84N14O36S3/1457.38 | 3810-74-0 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 100 g of Streptomycin sulfate to the solution.
- Add distilled water until the volume is 1 L.
- Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. Sterilize streptomycin stock through the prepared 0.22 µm syringe filter. Stock may be kept at -20°C for 1 year. Working Conc. = 100 µg/mL