To prepare L Dithiothreitol (DTT) (1 M) Stock Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
DL-Dithiothreitol | 154.25 g | 1 M | C4H10O2S2/154.25 | 3483-12-3 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 154.25 g of DL-Dithiothreitol to the solution.
- Add distilled water until the volume is 1 L.
- Store in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.