Dithiothreitol (DTT) (1 M) Stock Solution Preparation and Recipe

To prepare L  Dithiothreitol (DTT) (1 M) Stock Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
DL-Dithiothreitol154.25 g1 MC4H10O2S2/154.253483-12-3
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 154.25 g of DL-Dithiothreitol to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Store in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.