Zyxin antibody

Cat.#: 107549

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Product Information

  • Product Name
    Zyxin antibody
  • Documents
  • Description
    Zyxin Mouse Monoclonal antibody. Positive WB detected in HeLa cells, A431 cells, MCF-7 cells. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue, human breast cancer tissue, human cervical cancer tissue, human placenta tissue, human testis tissue. Observed molecular weight by Western-blot: 78kd
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse; other species not tested.
  • Alternative names
    ESP 2 antibody; HED 2 antibody; ZYX antibody; zyxin antibody; Zyxin 2 antibody
  • Immunogen
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of Zyxin recombinant protein (Accession Number: NM_001010972). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107549(ZYX antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107549(ZYX antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50

    Immunofluorescent analysis of HeLa cells using Catalog No:107549(ZYX Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

    Immunofluorescent analysis of HeLa cells using Catalog No:107549(ZYX Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG

  • Background
    Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"