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Product Name
Zyxin antibody
- Documents
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Description
Zyxin Mouse Monoclonal antibody. Positive WB detected in HeLa cells, A431 cells, MCF-7 cells. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue, human breast cancer tissue, human cervical cancer tissue, human placenta tissue, human testis tissue. Observed molecular weight by Western-blot: 78kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
ESP 2 antibody; HED 2 antibody; ZYX antibody; zyxin antibody; Zyxin 2 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Zyxin recombinant protein (Accession Number: NM_001010972). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107549(ZYX antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:107549(ZYX Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:107549(ZYX Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
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