ZP3 Polyclonal Antibody

Cat.#: 161482

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Product Information

  • Product Name
    ZP3 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ZP3
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ZP3 antibody; ZP3A antibody; ZP3B antibody; ZPC antibody; Zp-3 antibody; zona pellucida glycoprotein 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ZP3 (NP_001103824.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - ZP3 Polyclonal Antibody

    Western blot - ZP3 Polyclonal Antibody

    Western blot analysis of extracts of HeLa cells, using ZP3 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence - ZP3 Polyclonal Antibody

    Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"