ZP1 Polyclonal Antibody

Cat.#: 160614

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Product Information

  • Product Name
    ZP1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ZP1
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ZP1 antibody; HEL163 antibody; OOMD antibody; OOMD1 antibody; zona pellucida glycoprotein 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 340-610 of human ZP1 (NP_997224.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:100
  • Validations

    Western blot - ZP1 Polyclonal Antibody

    Western blot - ZP1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ZP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"