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Product Name
ZNF281 Polyclonal Antibody
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Description
Polyclonal antibody to ZNF281
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ZNF281 antibody; ZBP-99 antibody; ZNP-99 antibody; zinc finger protein 281 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 676-895 of human ZNF281 (NP_036614.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:3000
IHC 1:50 - 1:200 -
Validations
Western blot - ZNF281 Polyclonal Antibody
Western blot analysis of extracts of mouse spleen, using ZNF281 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.
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