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Product Name
ZNF217 Polyclonal Antibody
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Description
Polyclonal antibody to ZNF217
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ZNF217 antibody; ZABC1 antibody; zinc finger protein 217 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ZNF217 (NP_006517.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - ZNF217 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ZNF217 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - ZNF217 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ZNF217 antibody .
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Background
Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"