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Product Name
ZNF148 Polyclonal Antibody
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Description
Polyclonal antibody to ZNF148
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ZNF148 antibody; BERF-1 antibody; BFCOL1 antibody; GDACCF antibody; HT-BETA antibody; ZBP-89 antibody; ZFP148 antibody; pHZ-52 antibody; zinc finger protein 148 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-794 of human ZNF148 (NP_068799.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100 -
Validations
Western blot - ZNF148 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ZNF148 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human smooth muscle using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNF148 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ZNF148 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ZNF148 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using ZNF148 antibody .
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Background
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"