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Product Name
ZHX1 antibody
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Description
ZHX1 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive WB detected in HeLa cells, HEK-293 cells, mouse heart tissue, mouse kidney tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 100-120 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ZHX1 antibody; zinc fingers and homeoboxes 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZHX1 recombinant protein (Accession Number: NM_007222). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117052(ZHX1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:117052(ZHX1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:117052(ZHX1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ZHX1 (IP:Catalog No:117052, 4ug; Detection:Catalog No:117052 1:500) with HEK-293 cells lysate 3200ug.
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Background
Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress ranscription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128].
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