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Product Name
ZFYVE16 Polyclonal Antibody
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Description
Polyclonal antibody to ZFYVE16
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
ZFYVE16 antibody; PPP1R69 antibody; zinc finger FYVE-type containing 16 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE16 (NP_055548.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ZFYVE16 Polyclonal Antibody
Western blot analysis of extracts of mouse kidney, using ZFYVE16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - ZFYVE16 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ZFYVE16 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"