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Product Name
ZFYVE1 Polyclonal Antibody
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Description
Polyclonal antibody to ZFYVE1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ZFYVE1 antibody; DFCP1 antibody; PPP1R172 antibody; SR3 antibody; TAFF1 antibody; ZNFN2A1 antibody; zinc finger FYVE-type containing 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE1 (NP_067083.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ZFYVE1 Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using ZFYVE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - ZFYVE1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using ZFYVE1 antibody . Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"