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Product Name
ZEB1 antibody
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Description
ZEB1 Mouse Monoclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 190-200 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human; other species not tested.
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Alternative names
AREB6 antibody; BZP antibody; DELTA EF1 antibody; delta EF1 antibody; Negative regulator of IL2 antibody; NIL 2 A antibody; NIL 2 A zinc finger protein antibody; NIL 2A antibody; NIL2A antibody; TCF 8 antibody; TCF8 antibody; Transcription factor 8 antibody; ZEB antibody; ZEB1 antibody; ZFHEP antibody; ZFHX1A antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of ZEB1 recombinant protein (Accession Number: NM_001174096). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107548(ZEB1 Antibody) at dilution of 1:1000
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Background
ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"