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Product Name
ZBED1 Polyclonal Antibody
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Description
Polyclonal antibody to ZBED1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
ZBED1 antibody; ALTE antibody; DREF antibody; TRAMP antibody; hDREF antibody; zinc finger BED-type containing 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ZBED1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ZBED1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - ZBED1 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using ZBED1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"