• Z-DEVD-FMK peptide

Z-DEVD-FMK peptide

Not For Human Use, Lab Use Only.

Cat.#: 319064

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Special Price 286.2 USD

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Product Information

  • Product Name
    Z-DEVD-FMK peptide
  • Documents
  • Sequence Shortening
    DEVD, Asp-1 = Z-Asp(OMe), Glu-2 = Glu(OMe), Asp-4 = (OMe)-fluoromethylketone
  • Sequence
    Benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone
  • Length (aa)
    4
  • Peptide Purity (HPLC)
    95.62%
  • Molecular Formula
    C30H41FN4O12
  • Molecular Weight
    668.67
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Z-DEVD-FMK is a cell-permeable, irreversible inhibitor of caspase-3 with an IC50 of 0.13 µM and no activity at cathepsin B and cathepsin L. Z-DEVD-FMK inhibits apoptotic cell death in many cell types including cancer cells, it is neuroprotective in rat hippocampus following seizures in vivo and local application of Z-DEVD-FMK after spinal cord injury in rats reduces secondary tissue injury and helps preserve motor function.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Ma et al (1998) Synergistic effects of caspase inhibitors and MK-801 in brain injury after transient focal cerebral ischaemia in mice. Br J Pharmacol. 124(4) 756 PMID: 9690868
    • Henshall et al (2000) Involvement of caspase-3-like protease in the mechanism of cell death following focally evoked limbic seizures. J.Neurochem. 74 1215 PMID: 10693954
    • Barut et al (2005) The neuroprotective effects of z-DEVD.fmk, a caspase-3 inhibitor, on traumatic spinal cord injury in rats. Surg Neurol. 64(3) 213 PMID: 16099247
  • About TFA salt

    Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

    TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

    TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

    In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"