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YY1 antibody

Cat.#: 107545

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Product Information

  • Product Name
    YY1 antibody
  • Documents
  • Description
    YY1 Mouse Monoclonal antibody. Positive WB detected in C6 cells, COS-7 cells, HeLa cells, NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 68 kDa
  • Tested applications
    ELISA, IP, WB, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    DELTA antibody; Delta transcription factor antibody; INO80 complex subunit S antibody; INO80S antibody; NF E1 antibody; UCRBP antibody; Yin and yang 1 antibody; YIN YANG 1 antibody; YY 1 antibody; YY1 antibody; YY1 transcription factor antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of YY1 recombinant protein (Accession Number: NM_003403). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:5000-1:50000

    IP: 1:500-1:5000

    IF: 1:50-1:500

  • Validations

    Western blot of YY1 in various human cell lines with Catalog No:117348 at dilution of 1:50000.

    Western blot of YY1 in various human cell lines with Catalog No:117348 at dilution of 1:50000.

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with Catalog No:117348 at dilution of 1:50000.

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with Catalog No:117348 at dilution of 1:50000.

    IP Result of anti-YY1 (IP:Catalog No:117348, 5ug; Detection:Catalog No:117348 1:1000) with NIH/3T3 cells lysate 4000ug.

    IP Result of anti-YY1 (IP:Catalog No:117348, 5ug; Detection:Catalog No:117348 1:1000) with NIH/3T3 cells lysate 4000ug.

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:117348(YY1 Antibody) at dilution of 1:400 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:117348(YY1 Antibody) at dilution of 1:400 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65–68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"