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Product Name
YTHDF3-specific antibody
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Description
YTHDF3-specific Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive WB detected in HepG2 cells, A549 cells, HeLa cells, HL-60 cells, mouse liver tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ31657 antibody; YTH domain family protein 3 antibody; YTH domain family antibody; member 3 antibody; YTHDF3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YTHDF3-specific recombinant protein (Accession Number: NM_152758). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116896(YTHDF3 Antibody) at dilution of 1:600
Immunofluorescent analysis of A549 cells using Catalog No:116896(YTHDF3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"