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Product Name
YTHDC2 Polyclonal Antibody
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Description
Polyclonal antibody to YTHDC2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
YTHDC2 antibody; CAHL antibody; YTH domain containing 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - YTHDC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using YTHDC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - YTHDC2 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using YTHDC2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability. Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (By similarity). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease. Required for both spermatogenesis and oogenesis (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"