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Product Name
YARS Polyclonal Antibody
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Description
Polyclonal antibody to YARS
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
YARS antibody; CMTDIC antibody; TYRRS antibody; YRS antibody; YTS antibody; tyrosyl-tRNA synthetase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:200 -
Validations
Western blot - YARS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using YARS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - YARS Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug YARS antibody . Western blot was performed from the immunoprecipitate using YARS antibody at a dilition of 1:1000.
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Background
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"