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Product Name
XRN2 antibody
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Description
XRN2 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, COLO 320 cells, HeLa cells. Observed molecular weight by Western-blot: 104kd
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Tested applications
ELISA, WB
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
XRN2 antibody; DHM1 like protein antibody; DHP protein antibody; 5' 3' exoribonuclease 2 antibody; 5 3 exoribonuclease 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of XRN2 recombinant protein (Accession Number: BC006417). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116878(XRN2 antibody) at dilution of 1:200
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Background
XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
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References
- Wagschal A, Rousset E, Basavarajaiah P. Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII. Cell. 150(6):1147-57. 2012.
- Li Y, Yamane D, Lemon SM. Dissecting the roles of the 5' exoribonucleases Xrn1 and Xrn2 in restricting hepatitis C virus replication. Journal of virology. 89(9):4857-65. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"