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Product Name
XRN1 antibody
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Description
XRN1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue, human brain tissue. Positive WB detected in HeLa cells, Neuro-2a cells. Observed molecular weight by Western-blot: 175 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
5 3 exoribonuclease 1 antibody; 5' 3' exoribonuclease 1 antibody; DKFZp434P0721 antibody; DKFZp686B22225 antibody; DKFZp686F19113 antibody; FLJ41903 antibody; SEP1 antibody; XRN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of XRN1 recombinant protein (Accession Number: BC078146). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116877(XRN1 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:116877(XRN1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:116877(XRN1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:116877(XRN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.
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