XPO5 Polyclonal Antibody

Cat.#: 165042

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Product Information

  • Product Name
    XPO5 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to XPO5
  • Tested applications
    WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    XPO5 antibody; exp5 antibody; exportin-5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 925-1204 of human XPO5 (NP_065801.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:100
    IP 1:50 - 1:200
  • Validations

    Western blot - XPO5 Polyclonal Antibody

    Western blot - XPO5 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using XPO5 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - XPO5 Polyclonal Antibody

    Immunohistochemistry - XPO5 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human oophoroma using XPO5 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - XPO5 Polyclonal Antibody

    Immunohistochemistry - XPO5 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse jejunum using XPO5 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - XPO5 Polyclonal Antibody

    Immunofluorescence - XPO5 Polyclonal Antibody

    Immunofluorescence analysis of MCF7 cells using XPO5 antibody .

    Immunoprecipitation - XPO5 Polyclonal Antibody

    Immunoprecipitation - XPO5 Polyclonal Antibody

    Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug XPO5 antibody . Western blot was performed from the immunoprecipitate using XPO5 antibody at a dilition of 1:1000.

  • Background
    Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.; Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.; (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"