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Product Name
XPA Polyclonal Antibody
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Description
Polyclonal antibody to XPA
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
XPA antibody; XP1 antibody; XPAC antibody; DNA repair protein complementing XP-A cells antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - XPA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using XPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - XPA Polyclonal Antibody
Western blot analysis of extracts from normal (control) and XPA knockout HeLa cells, using XPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunofluorescence - XPA Polyclonal Antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody . Greenuff1aGFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence - XPA Polyclonal Antibody
Immunofluorescence analysis of A549 cells using XPA antibody .
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Background
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"