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WWP1 antibody

Cat.#: 116978

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Product Information

  • Product Name
    WWP1 antibody
  • Documents
  • Description
    WWP1 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 95 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AIP5 antibody; DKFZp434D2111 antibody; hSDRP1 antibody; Tiul1 antibody; WW domain containing protein 1 antibody; WWP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of WWP1 recombinant protein (Accession Number: XM_005250760). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IF: 1:20-1:200

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116978(WWP1 antibody) at dilution of 1:300

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:116978(WWP1 antibody) at dilution of 1:300

    IP Result of anti-WWP1 (IP:Catalog No:116978, 4ug; Detection:Catalog No:116978 1:300) with PC-3 cells lysate 1040ug.

    IP Result of anti-WWP1 (IP:Catalog No:116978, 4ug; Detection:Catalog No:116978 1:300) with PC-3 cells lysate 1040ug.

    Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:116978(WWP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:116978(WWP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.
  • References
    • Zhang XF, Chao J, Pan QZ. Overexpression of WWP1 promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma. Oncotarget. 6(38):40920-33. 2015.
    • Lee JO, Lee SK, Kim N. E3 ubiquitin ligase, WWP1, interacts with AMPKα2 and down-regulates its expression in skeletal muscle C2C12 cells. The Journal of biological chemistry. 288(7):4673-80. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"