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Product Name
WWC2 antibody
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Description
WWC2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human testis tissue. Positive IF detected in HEK-293 cells. Positive WB detected in mouse brain tissue, HepG2 cells. Observed molecular weight by Western-blot: 134 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BH 3 only member B antibody; BOMB antibody; FLJ22029 antibody; FLJ34082 antibody; Protein WWC2 antibody; WW and C2 domain containing 2 antibody; WW domain containing protein 2 antibody; WWC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WWC2 recombinant protein (Accession Number: BC017957). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116976(WWC2 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:116976(WWC2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116976(WWC2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:116976(WWC2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
WWC2 alson named as BOMB or WW and C2 domain containing 2 is 1192 amino acid protein, which contains 1 C2 domain and 2 WW domains. WWC2 localizes in cytoplasm and belongs to the WWC family. , WWC2 exists as seven alternatively spliced isoforms and is encoded by a gene located on human chromosome 4q35.1. WWC2 may be expressed in liver, brain, prostate and kidney. The function of WWC2 is still non-known and need to be further studied.
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