WRAP53 antibody

Cat.#: 116839

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Product Information

  • Product Name
    WRAP53 antibody
  • Documents
  • Description
    WRAP53 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Observed molecular weight by Western-blot: 72-75kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ10385 antibody; TCAB1 antibody; WDR79 antibody; WRAP53 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of WRAP53 recombinant protein (Accession Number: NM_001143990). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116839(WRAP53 antibody) at dilution of 1:300

    human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116839(WRAP53 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-WRAP53 (IP:Catalog No:116839, 4ug; Detection:Catalog No:116839 1:300) with HeLa cells lysate 2480ug.

    IP Result of anti-WRAP53 (IP:Catalog No:116839, 4ug; Detection:Catalog No:116839 1:300) with HeLa cells lysate 2480ug.

  • Background
    WRAP53 represents WD repeat containing, antisense to TP53
  • References
    • Rao X, Huang D, Sui X. Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma. PloS one. 9(3):e91670. 2014.
    • Henriksson S, Rassoolzadeh H, Hedström E. The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes & development. 28(24):2726-38. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"