WNT9A Polyclonal Antibody

Cat.#: 165622

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Product Information

  • Product Name
    WNT9A Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to WNT9A
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    WNT9A antibody; WNT14 antibody; protein Wnt-9a antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:100 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - WNT9A Polyclonal Antibody

    Western blot - WNT9A Polyclonal Antibody

    Western blot analysis of extracts of mouse lung, using WNT9A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry - WNT9A Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - WNT9A Polyclonal Antibody

    Immunofluorescence - WNT9A Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using WNT9A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"