-
Product Name
WNT5B Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to WNT5B
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
WNT5B antibody; protein Wnt-5b antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - WNT5B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using WNT5B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis leydig using WNT5B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WNT5B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using WNT5B antibody at dilution of 1:100 (40x lens).
-
Background
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"