WNT3 antibody

Cat.#: 116835

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Product Information

  • Product Name
    WNT3 antibody
  • Documents
  • Description
    WNT3 Rabbit Polyclonal antibody. Positive WB detected in SGC-7901 cells, NIH/3T3 cells, Raji cells, SH-SY5Y cells. Positive IP detected in MCF-7 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 45-50 kDa
  • Tested applications
    ELISA, WB, FC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    INT4 antibody; Proto oncogene Int 4 homolog antibody; Proto oncogene Wnt 3 antibody; WNT3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of WNT3 recombinant protein (Accession Number: NM_030753). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

  • Validations

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:116835(WNT3 antibody) at dilution of 1:600

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:116835(WNT3 antibody) at dilution of 1:600

    IP Result of anti-WNT3 (IP:Catalog No:116835, 4ug; Detection:Catalog No:116835 1:500) with MCF-7 cells lysate 3200ug.

    IP Result of anti-WNT3 (IP:Catalog No:116835, 4ug; Detection:Catalog No:116835 1:500) with MCF-7 cells lysate 3200ug.

    1X10^6 HeLa cells were stained with 0.2ug WNT3 antibody (Catalog No:116835, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with 0.2ug WNT3 antibody (Catalog No:116835, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    WNT3 represents wingless-type MMTV integration site family, member 3

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"