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Product Name
WNT2 Polyclonal Antibody
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Description
Polyclonal antibody to WNT2
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
WNT2 antibody; INT1L1 antibody; IRP antibody; protein Wnt-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-360 of human WNT2 (NP_003382.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - WNT2 Polyclonal Antibody
Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - WNT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using WNT2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - WNT2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using WNT2 antibody at dilution of 1:200 (40x lens).
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Background
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Functions as upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"