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Product Name
WNT10B Polyclonal Antibody
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Description
Polyclonal antibody to WNT10B
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
WNT10B antibody; SHFM6 antibody; STHAG8 antibody; WNT-12 antibody; protein Wnt-10b antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant Protein of human WNT10B
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - WNT10B Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using WNT10B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"