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Product Name
WHSC1 Polyclonal Antibody
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Description
Polyclonal antibody to WHSC1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
NSD2 antibody; KMT3F antibody; KMT3G antibody; MMSET antibody; REIIBP antibody; TRX5 antibody; WHS antibody; WHSC1 antibody; histone-lysine N-methyltransferase NSD2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human WHSC1 (NP_579877.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - WHSC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using WHSC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - WHSC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using WHSC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WHSC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using WHSC1 antibody at dilution of 1:100 (40x lens).
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Background
Histone methyltransferase with histone H3 'Lys-27' (H3K27me) methyltransferase activity. Isoform 2 may act as a transcription regulator that binds DNA and suppresses IL5 transcription through HDAC recruitment.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"