WEE1 Polyclonal Antibody

Cat.#: 163115

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Product Information

  • Product Name
    WEE1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to WEE1
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    WEE1 antibody; WEE1A antibody; WEE1hu antibody; wee1-like protein kinase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human WEE1
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - WEE1 Polyclonal Antibody

    Western blot - WEE1 Polyclonal Antibody

    Western blot analysis of extracts of HeLa cells, using WEE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

  • Background
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"