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Product Name
WDR77 Monoclonal Antibody
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Description
Monoclonal antibody to WDR77
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Tested applications
WB, ICC
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Species reactivity
Human, Mouse, Rat
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Alternative names
WDR77 antibody; HKMT1069 antibody; MEP-50 antibody; MEP50 antibody; Nbla10071 antibody; p44 antibody; p44/Mep50 antibody; WD repeat domain 77 antibody
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Isotype
Mouse IgG
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Preparation
Antigen: Recombinant protein of human WDR77
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
ICC 1:50 - 1:200 -
Validations
Western blot - WDR77 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"