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Product Name
WDR55 antibody
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Description
WDR55 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon tissue. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 42-50 kDa
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Tested applications
ELISA, IF, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ20195 antibody; FLJ21702 antibody; WD repeat domain 55 antibody; WDR55 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR55 recombinant protein (Accession Number: BC002482). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human colon using Catalog No:116812(WDR55 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using WDR55 antibody Catalog No:116812 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116812(WDR55 Antibody) at dilution of 1:300
IP Result of anti-WDR55 (IP:Catalog No:116812, 4ug; Detection:Catalog No:116812 1:300) with HeLa cells lysate 3440ug.
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Background
WD repeat domain 55 (WDR55) also named as, FLJ20195, is a 383 amino acid protein, which contains seven WD repeats and belongs to the WD repeat WDR55 family. WDR55 localizes in the nucleus and cytoplasm. WDR55 acts as a modulator of rRNA synthesis and play a central role in organogenesis.
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