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Product Name
WDR40A antibody
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Description
WDR40A Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue, human lung cancer tissue. Positive WB detected in HEK-293 cells, Jurkat cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IF, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DCAF12 antibody; DKFZp434O125 antibody; KIAA1892 antibody; TCC52 antibody; WD repeat domain 40A antibody; WDR40A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR40A recombinant protein (Accession Number: BC008893). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of Hela cells, using WDR40A antibody Catalog No:116867 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116867(WDR40A Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116867(WDR40A Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116867(WDR40A Antibody) at dilution of 1:50 (under 40x lens)
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Background
WDR40A, also known as DCAF12, is a 51 kDa protein. It may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex(PMID: 16949367). This antibody specifically recognises the 51 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"