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Product Name
WDR19/IFT144 antibody
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Description
WDR19/IFT144 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human prostate hyperplasia tissue, human prostate cancer tissue. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells. Observed molecular weight by Western-blot: 145-152kd
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ23127 antibody; IFT144 antibody; KIAA1638 antibody; ORF26 antibody; PWDMP antibody; WD repeat domain 19 antibody; WDR19 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR19/IFT144 recombinant protein (Accession Number: BC032578). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116858(WDR19 antibody) at dilution of 1:600
IF result (ocalization near the Fleming body) of anti-WDR19 (Catalog No:116858, 1:50) with telophase hTERT-RPE1 cells (MeOH fixed) by Dr. Moshe Kim.
IP Result of anti-WDR19 (IP:Catalog No:116858, 4ug; Detection:Catalog No:116858 1:500) with HEK-293 cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using Catalog No:116858 (WDR19 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using Catalog No:116858 (WDR19 Antibody) at dilution of 1:50
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Background
WDR19 represents WD repeat domain 19
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References
- Luo N, Conwell MD, Chen X. Primary cilia signaling mediates intraocular pressure sensation. Proceedings of the National Academy of Sciences of the United States of America. 111(35):12871-6. 2014.
- Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"