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Product Name
WBSCR22 Polyclonal Antibody
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Description
Polyclonal antibody to WBSCR22
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
BUD23 antibody; HASJ4442 antibody; HUSSY-3 antibody; MERM1 antibody; PP3381 antibody; WBMT antibody; WBSCR22 antibody; probable 18S rRNA (guanine-N)-methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human WBSCR22 (NP_059998.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - WBSCR22 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using WBSCR22 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - WBSCR22 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using WBSCR22 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - WBSCR22 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using WBSCR22 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - WBSCR22 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using WBSCR22 antibody .
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Background
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors. Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements. Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"