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Product Name
VSTM1 antibody
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Description
VSTM1 Rabbit Polyclonal antibody. Positive FC detected in HL-60 cells. Positive WB detected in HL-60 cells. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, FC
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Species reactivity
Human; other species not tested.
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Alternative names
UNQ3033 antibody; VSTM1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VSTM1 recombinant protein (Accession Number: XM_017026666). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:116801(VSTM1 Antibody) at dilution of 1:300
1X10^6 HL-60 cells were stained with 0.2ug VSTM1 antibody (Catalog No:116801, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
VSTM1 (V-set and transmembrane domain-containing protein 1) has two main splicing isoform, VSTM1-v1 and VSTM1-v2. VSTM1-v1 is a type I membrane molecule, mainly expressed on human peripheral blood granulocytes and monocytes. VSTM1-v2 is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells. (PMID: 22960280; 23909423)
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