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Product Name
VPS8 antibody
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Description
VPS8 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 151 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ32099 antibody; KIAA0804 antibody; VPS8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS8 recombinant protein (Accession Number: BC001001). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116797(VPS8 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using VPS8 antibody Catalog No:116797 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-VPS8 (IP:Catalog No:116797, 4ug; Detection:Catalog No:116797 1:600) with mouse brain tissue lysate 4000ug.
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Background
VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.
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References
- Perini ED, Schaefer R, Stöter M, Kalaidzidis Y, Zerial M. Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations. Traffic (Copenhagen, Denmark). 15(12):1366-89. 2014.
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