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Product Name
VPS54 Polyclonal Antibody
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Description
Polyclonal antibody to VPS54
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
VPS54 antibody; HCC8 antibody; PPP1R164 antibody; SLP-8p antibody; VPS54L antibody; WR antibody; hVps54L antibody; VPS54, GARP complex subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_001005739.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - VPS54 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using VPS54 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"