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Product Name
VPS53 antibody
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Description
VPS53 Rabbit Polyclonal antibody. Positive WB detected in human liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 95-97 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10979 antibody; HCCS1 antibody; hVps53L antibody; pp13624 antibody; VPS53 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS53 recombinant protein (Accession Number: BC029560). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116794(VPS53 antibody) at dilution of 1:300
IP Result of anti-VPS53 (IP:Catalog No:116794, 4ug; Detection:Catalog No:116794 1:500) with mouse liver tissue lysate 4000ug.
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Background
VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"