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Product Name
VPS52 antibody
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Description
VPS52 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive IP detected in mouse skin tissue. Positive WB detected in HepG2 cells, COLO 320 cells, mouse skin tissue. Observed molecular weight by Western-blot: 82kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARE1 antibody; dJ1033B10.5 antibody; DKFZp547I194 antibody; RP5 1033B10 antibody; SAC2 antibody; SACM2L antibody; VPS52 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS52 recombinant protein (Accession Number: NM_022553). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116793(VPS52 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116793(VPS52 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-VPS52 (IP:Catalog No:116793, 3ug; Detection:Catalog No:116793 1:1000) with mouse skin tissue lysate 3200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116793(VPS52 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).
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