VPS45 antibody

Cat.#: 116789

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Product Information

  • Product Name
    VPS45 antibody
  • Documents
  • Description
    VPS45 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human heart tissue, human testis tissue. Positive IF detected in HepG2 cells.
  • Tested applications
    ELISA, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    h VPS45 antibody; H1VPS45 antibody; hlVps45 antibody; VPS45 antibody; VPS45A antibody; VPS45B antibody; VPS54A antibody; VSP45 antibody; VSP45A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of VPS45 recombinant protein (Accession Number: NM_007259). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116789(VPS45 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116789(VPS45 antibody) at dilution of 1:50 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using VPS45 antibody Catalog No:116789 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using VPS45 antibody Catalog No:116789 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Background
    The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
  • References
    • Laufman O, Hong W, Lev S. The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. Journal of cell science. 126(Pt 6):1506-16. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"