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Product Name
VPS45 antibody
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Description
VPS45 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human heart tissue, human testis tissue. Positive IF detected in HepG2 cells.
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Tested applications
ELISA, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
h VPS45 antibody; H1VPS45 antibody; hlVps45 antibody; VPS45 antibody; VPS45A antibody; VPS45B antibody; VPS54A antibody; VSP45 antibody; VSP45A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS45 recombinant protein (Accession Number: NM_007259). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116789(VPS45 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using VPS45 antibody Catalog No:116789 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
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References
- Laufman O, Hong W, Lev S. The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. Journal of cell science. 126(Pt 6):1506-16. 2013.
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