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Product Name
VPS41 antibody
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Description
VPS41 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 94kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
HVPS41 antibody; hVps41p antibody; HVSP41 antibody; S53 antibody; VPS41 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS41 recombinant protein (Accession Number: NM_014396). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116788(VPS41 antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells using Catalog No:116788(VPS41 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. VPS41 (vacuolar protein sorting 41) is the human ortholog of yeast Vps41 protein. Yeast Vps41 is a subunit of AP3 (heterotetrameric adaptor protein complex 3) and HOPS (homotypic fusion and protein transport) complexes and is involved in trafficking of proteins from the late Golgi to the vacuole. In mammalian cells, Vps41 was found to be a member of HOPS complex, mediating the trafficking of proteins to lysosomes (PMID: 23173123). It has been reported that human VPS41 has a HOPS-independent function in the LAMP carrier pathway and also participates in lysosomal maturation, probably as part of the HOPS complex (PMID: 23322049).
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References
- van der Kant R, Fish A, Janssen L. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. Journal of cell science. 126(Pt 15):3462-74. 2013.
- van der Kant R, Jonker CT, Wijdeven RH. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. The Journal of biological chemistry. 290(51):30280-90. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"